urovestВестник урологииUrology Herald2308-6424Rostov State Medical University10.21886/2308-6424-2021-9-3-5-11urovest-466Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИзменения фенотипа канальцевого эпителия нефрона контрлатеральной почки при односторонней непроходимости мочеточника (экспериментальное исследование)The changes of the tubular epithelium phenotype in the contralateral kidney nephrons while developing unilateral ureteral obstruction: an experimental studyhttps://orcid.org/0000-0001-8792-6911АкименкоМ. А.AkimenkoM. A.

Марина Анатольевна Акименко – аспирант кафедры медицинской биологии и генетики

344022, г. Ростов-на-Дону, пер. Нахичеванский, д. 29

тел.: +7 (928) 187-79-84

Marina A. Akimenko – Postgraduate student, Dept. of Medical Biology and Genetics

344022, Rostov-on-Don, 29 Nakhichevanskiy ln.

tel.: +7 (928) 187-79-84

akimenkoma@yandex.ruhttps://orcid.org/0000-0003-0542-6900ВороноваО. В.VoronovaO. V.

Ольга Владимировна Воронова – ассистент кафедры судебной медицины

344022, г. Ростов-на-Дону, пер. Нахичеванский, д. 29

Olga V. Voronova – Assist.; Dept. of Forensic Medicine

344022, Rostov-on-Don, 29 Nakhichevanskiy ln.

9043401873@mail.ruhttps://orcid.org/0000-0002-1923-3168КолмаковаТ. С.KolmakovaT. S.

Татьяна Сергеевна Колмакова – докт. биол. наук, доцент; заведующая кафедрой медицинской биологии и генетики

344022, г. Ростов-на-Дону, пер. Нахичеванский, д. 29

Tatyana  S.  Kolmakova  –  Dr.Sc.(Biol.),  Assoc.  Prof.;  Head, Dept. of Medical Biology and Genetics

344022, Rostov-on-Don, 29 Nakhichevanskiy ln.

tat_kolmakova@mail.ruФГБОУ ВО «Ростовский государственный медицинский университет» Минздрава РоссииРоссияRostov State Medical UniversityRussian Federation20210410202193511Copyright © Акименко М.А., Воронова О.В., Колмакова Т.С., 20212021Акименко М.А., Воронова О.В., Колмакова Т.С.Akimenko M.A., Voronova O.V., Kolmakova T.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.urovest.ru/jour/article/view/466Введение

Введение. Широкое распространение заболеваний почек, вызванных обструкцией мочевых путей, привело к необходимости экспериментальных исследований закономерностей развития компенсаторных и патологических процессов при их поражении. Актуальным является изучение ключевых механизмов, обеспечивающих выполнение компенсаторных функций контрлатеральной почкой, для ранней диагностики, лечения и прогноза обструктивных заболеваний почек.

Цель исследования

Цель исследования. Изучить изменения фенотипа эпителиальных клеток нефрона контрлатеральной почки при односторонней непроходимости мочеточника на экспериментальной модели.

Материалы и методы

Материалы и методы. Модель односторонней непроходимости мочеточника воспроизведена на взрослых кроликах. Исследования проведены на 7-е, 14-е и 21-е сутки полной обструкции левого мочеточника. Выполнено иммунофенотипирование образцов ткани контрлатеральной почки на маркеры эпителиального фенотипа (цитокератин 7, Е-кадгерин) и мезенхимального фенотипа (виментин, гладкомышечный актин).

Результаты

Результаты. Контрлатеральная почка при дополнительной нагрузке способна длительно сохранять морфофункциональные характеристики нефрона. Первые признаки изменения фенотипа эпителия нефрона зарегистрированы на 21-е сутки в виде появления диффузной визуализации маркера мезенхимального фенотипа (виментина), на фоне абсолютного сохранения визуализации маркеров эпителиального фенотипа.

Заключение

Заключение. Полученные результаты позволяют полагать, что при увеличении срока обструкции адаптационный резерв контрлатеральной почки постепенно снижается, что повышает риск развития событий по негативному сценарию.

Introduction

Introduction. The high prevalence of renal diseases caused by urinary tract obstruction led to the need for experimental research of compensatory and pathological processes with kidney injury. It is also of relevance to study key mechanisms providing a compensatory function of the contralateral kidney for early diagnosis, treatment, and prognosis of obstructive renal diseases.

Purpose of the study

Purpose of the study. To examine epithelial nephron cells phenotype dynamics changes in contralateral kidney using unilateral ureteral obstruction experimental model.

Materials and methods

Materials and methods. Model of unilateral ureteral obstruction was established using adult rabbits. The studies were carried out on days 7, 14 and 21 of complete obstruction of the left ureter. Immunophenotyping was performed on contralateral kidney tissue samples using epithelial (cytokeratin 7, E-cadherin) and mesenchymal (vimentin, α-smooth muscle actin) markers.

Results

Results. The contralateral kidney under additional load can maintain the morphological and functional characteristics of the nephron for a long time. The first transmogrify signs in the nephron epithelium phenotype were detected by day 21 as the diffuse appearance of mesenchymal marker vimentin with unaltered visualization of epithelial phenotype markers.

Conclusion

Conclusion. The results obtained allow us to assume that the compensatory reserve of the contralateral kidney is gradually decreasing when the duration of the obstruction increases. Thus, the likelihood of developing negative disorders increases.

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The authors declare that there are no conflicts of interest present.